Journal: Cell reports

Article Title: Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development

doi: 10.1016/j.celrep.2020.108448

Figure Lengend Snippet: (A) Quantification of miRNAs from a 3D culture of mature human microvessels with human umbilical vein endothelial cells (HUVECs) irradiated with 0.5 Gy of simplified simulated galactic cosmic rays (SimGCRSim) compared to sham irradiated samples 48 h after irradiation. All candidate miRNAs were examined, but only the miRNAs that seemed to respond differently for each group are shown. The error bars represent SEM. (B and C) Mature microvessels fixed and fluorescently stained with 5-(4,6-dichlorotriazinyl) aminofluorescein (DTAF) 48 h after SimGCRSim irradiation with or without antagomir-induced inhibition of miR-125b, miR-16, and let-7a starting at 24 h prior to irradiation. Scrambled version of the antagomir was used a vehicle control. (D) Summary of all the fold-change values for all experiments utilized in this manuscript (including both ddPCR and miRNA-seq data). (E) The summary of the overall impact of the miRNAs on the gene targets determined in through pathway analysis on the miRNA-seq data. The arrows indicate the degree of up- or downregulation, and the colors of the arrows indicate the significance (i.e., p values). The gray-shaded regions represent the 11-day time points after irradiation. The red-shaded miRNAs represent the miRNAs used for the antagomir experiments in (B) and (C).

Article Snippet: Irradiations were carried out at Brookhaven National Lab using 50cGy of SimGCRSim (5-ion simplified galactic cosmic ray simulation: protons at 1000MeV, 28 Si at 600 MeV/n, 4 He at 250 MeV/n, 16 O at 350 MeV/n, 56 Fe at 600 MeV/n, and protons at 250 MeV).

Techniques: Irradiation, Staining, Inhibition, Control